| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tri-Cities United Soccer Club Tri-Cities United 06/07 Boys Premier |
0106-011B5U-0001 | Ovidiu Chirica |
| A2 | Jackson Soccer Club JSC 2006 Boys |
0609-012B5U-0147 | Malang Jarju |
| A3 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 06B SL |
0324-022B5U-0010 | Christopher Zeller |
| A4 | Germantown Legends Germantown Legends 06 Black |
0606-012B5U-0013 | Shawn Loth |
| A5 | Knoxville Reds Football Club - 01 Knoxville Reds 06 Barcelona |
0290-01D2B5U-0008 | Gabriel Walker |
| A6 | Kings Hammer 2006 Elite Strikers |
0515-012B5U-0006 | Raul Lopez |
| A7 | Clarksville Soccer Club Clarksville SC - B2006 Maroon |
0422-012B5U-0005 | Scott Dollar |
| A8 | Memphis Futbol Club / Memphis Soccer Education Memphis FC 06 Boys Blue - SL |
0624-012B5U-2333 | Andres Flores |