| Group | Club/Team Name | Team ID | Head Coach |
| A1 | All-In FC TN All-In FC TN 05/06 Boys |
0443-01SLB6U-0045 | Aaron Alva |
| A2 | Tennessee United Soccer Club TUSC 2005 Boys White |
0424-013B6U-0041 | Bryson Lehn |
| A3 | McMinn United Futbol Club McMinn United Futbol Club - B16U |
0308-013B6U-2090 | Seth Rucker |
| A4 | Nashville United Soccer Academy - 01 NUSA B05 Navy |
0534-012B6U-0021 | Mirgen Bushati |