| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Wilson United Soccer Club EESC 2005 Boys Black |
0406-012B6U-1032 | Baylor McKay |
| A2 | Tennessee United Soccer Club TUSC 2005 Boys Blue |
0424-012B6U-0034 | Robert DeWitt |
| A3 | ALBION SC Nashville (Formerly Southern Premier Soccer) Southern Premier Soccer 05 Boys |
0540-02D2B6U-0019 | Brent Goulet |
| A4 | Knoxville FC (Crush) Knoxville FC Crush 05B Premier |
0206-012B6U-0009 | Vamuyan Sheriff |
| A5 | Aris FC Aris FC - B16U Red 2005 |
0530-022B6U-0003 | Andreas Petrou |
| A6 | Germantown Legends Germantown Legends 05 White |
0606-012B6U-0014 | A Carbognani - DO NOT USE |
| A7 | Knoxville Reds Football Club - 01 Knoxville Reds 05 Lions |
0290-01D2B6U-0011 | Patrick Fiveash |
| A8 | Tennessee Soccer Club TSC Williamson 2005 Boys Premier |
0522-012B6U-0139 | Craig Parker |