| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee Soccer Club TSC Murfreesboro 2004 Boys Showcase |
0522-011B7U-2009 | Jorge Suarez |
| A2 | Chattanooga FC Academy Chattanooga FC Academy 2004 Navy Boys |
0316-021B7U-7797 | Nickson Odeny |
| A3 | Tennessee Soccer Club TSC Williamson 2004 Boys Premier |
0522-012B7U-0145 | Christian Dale |
| A4 | Tri-Cities United Soccer Club Tri-Cities United 04/05 Boys Premier |
0106-011B7U-0002 | Matt Barry |
| A5 | Nashville United Soccer Academy - 01 NUSA B04 Navy |
0534-012B7U-0024 | Shane Keely |
| A6 | Upper Cumberland United Soccer Club - 01 UC United B 2004/2005 |
0426-012B7U-1510 | Jeremy Govero |
| A7 | Jackson Soccer Club JSC 2004 Boys |
0609-012B7U-0145 | Musa Manneh |
| A8 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 04B SL |
0324-021B7U-0012 | Nicholas Davis |