| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Germantown Legends Germantown Legends 04 Black NL |
0606-011B7U-0005 | Carey Talley |
| A2 | Nashville United Soccer Academy - 01 NUSA B04 Maroon |
0534-011B7U-0022 | Andy Poklad |
| A3 | Memphis Futbol Club / Memphis Soccer Education Memphis FC 04 Boys Blue - SL |
0624-011B7U-2332 | Carl Schmitt |
| A4 | Tennessee Soccer Club TSC Nashville 2004 Boys Showcase |
0522-011B7U-0092 | Anthony Torres |
| A5 | Tennessee Soccer Club TSC Williamson 2004 Boys Showcase |
0522-011B7U-0093 | Eric Bossman |
| A6 | Lobos Rush (Collierville Soccer Association) 04 Lobos Rush Premier |
0603-011B7U-0034 | Jordan Bradshaw |
| A7 | Emerald Force Soccer Club - 01 Emerald Force 04 Celtic |
0214-011B7U-0069 | Kelvin Scott |
| A8 | Tennessee United Soccer Club TUSC 2004 Boys Red |
0424-011B7U-0010 | A Morrell |